Advanced search
Publication Cover
Xenobiotica
the fate of foreign compounds in biological systems
Volume 37, 2007 - Issue 3
Submit an article Journal homepage
148
Views
8
CrossRef citations to date
0
Altmetric
Research Article

Identification of novel dioxin-responsive genes by representational difference analysis

S. P. Rivera Department of Pathology and Laboratory Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA
,
S. T. Saarikoski Department of Pathology and Laboratory Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA; Department of Mental Health and Alcohol Research, National Public Health Institute, FI-00250, Helsinki, Finland
,
W. Sun Department of Pathology and Laboratory Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA
&
O. Hankinson Department of Pathology and Laboratory Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA
Pages 271-279 | Received 17 Nov 2006, Accepted 13 Dec 2006, Published online: 22 Sep 2008
 
Sample our Medicine, Dentistry, Nursing & Allied Health journals, sign in here to start your FREE access for 14 days

Abstract

Representational difference analysis (RDA) was employed on dioxin-stimulated Hepa-1 mouse hepatoma cells (human breast cancer) MCF-7 cells, mouse liver, and mouse thymocytes in order to identify novel responder genes to dioxin. In addition to several clones representing known dioxin-inducible genes, several clones were isolated that represented genes that were previously not known to be inducible by dioxin, including B94 (also known as tumour necrosis factor alpha-induced protein 2 (Tnfaip2), Seven in absentia homologue 2 (Siah2), the mouse homologue of Bob/Gpr15, and S-adenosyl-homocysteine hydrolase (Sahh, Ahcy). Induction of these genes by dioxin in Hepa-1 cells was rapid. Furthermore, induction occurred in the presence of the protein synthesis inhibitor cycloheximide, indicating that in each case induction is a primary response.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.