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Xenobiotica
the fate of foreign compounds in biological systems
Volume 37, 2007 - Issue 3
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Research Article

Identification of novel dioxin-responsive genes by representational difference analysis

S. P. Rivera Department of Pathology and Laboratory Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA
,
S. T. Saarikoski Department of Pathology and Laboratory Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA; Department of Mental Health and Alcohol Research, National Public Health Institute, FI-00250, Helsinki, Finland
,
W. Sun Department of Pathology and Laboratory Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA
&
O. Hankinson Department of Pathology and Laboratory Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA
Pages 271-279 | Received 17 Nov 2006, Accepted 13 Dec 2006, Published online: 22 Sep 2008
 
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Abstract

Representational difference analysis (RDA) was employed on dioxin-stimulated Hepa-1 mouse hepatoma cells (human breast cancer) MCF-7 cells, mouse liver, and mouse thymocytes in order to identify novel responder genes to dioxin. In addition to several clones representing known dioxin-inducible genes, several clones were isolated that represented genes that were previously not known to be inducible by dioxin, including B94 (also known as tumour necrosis factor alpha-induced protein 2 (Tnfaip2), Seven in absentia homologue 2 (Siah2), the mouse homologue of Bob/Gpr15, and S-adenosyl-homocysteine hydrolase (Sahh, Ahcy). Induction of these genes by dioxin in Hepa-1 cells was rapid. Furthermore, induction occurred in the presence of the protein synthesis inhibitor cycloheximide, indicating that in each case induction is a primary response.

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