Novel metabolites of warfarin produced by Beauveria bassiana and Streptomyces rimosus : a novel application of hplc- nmr

Abstract

1. Biotransformation of warfarin by the fungus Beauveria bassiana (ATCC 7159) yielded the first reported phase II warfarin metabolite, 3′,4′-dihydroxywarfarin-3′-[4- methoxyglucoside], another previously unreported metabolite, 3′, 4′-dihydroxywarfarin and also 4′-hydroxywarfarin. 2. Biotransformation of warfarin by Streptomyces rimosus (NRRL 2234) yielded the previously unreported metabolites, 12-hydroxywarfarin,4′-hydroxy-11-methoxywarfarin, and also 7-hydroxywarfarin and 4′-hydroxywarfarin, which have not been previously reported as biotransformation products from this organism. 3. Hplc-nmr has been used to identify biotransformation products of warfarin by S. rimosus directly from the microbial broth without prior isolation and purification.