Synthesis of Sunflower Oil Based Bimetallic Polymer and Its Antifungal Studies

Abstract

Bimetallic oil based polymer was prepared by condensation polymerization reaction. FTIR and ¹H NMR have been used to support structure of the polymers. Thermal behavior of the polymer was established by thermogravimetry/differential thermal analysis (TG/DTA). The polymers were examined for their antifungal properties by performing minimum inhibitory concentration assessment, growth curve studies, and H⁺ extrusion studies using Candida species as model organisms. Growth and sensitivity of the organisms were significantly effected by test polymers at different concentrations. Insight studies to mechanism suggested that the synthesized polymers exerts their antifungal activity by targeting H⁺-ATPase mediated H⁺-pumping, inhibition of H⁺-ATPase leads to intracellular acidification and cell death.

Keywords:

• Antifungal
• bimetallic
• cell death
• intracellular acidification
• polyester amide resin
• sunflower oil

Acknowledgement

The authors are thankful to University Grant commission, India, for providing financial assistance; and Sumathi Muralidhar, from the Sexually Transmitted Lab, Safdarjang Hospital, New Delhi, India, for providing standard Candida species.

Notes

^a Seq No. 179 (invasive).

^b Seq No. 41 (respiratory).

^c Seq No. 78 (invasive).

^d Seq No. 80 (cutaneous).

^e Seq No. 1685 (respiratory).

The data (mean ± SD) represent three experiments. Solvent was the negative control, fluconazole was the positive control).

^a Seq No. 179 (invasive).

^b Seq No. 41 (respiratory).

^c Seq No. 78 (invasive).

^d Seq No. 80 (cutaneous).

^e Seq No. 1685 (respiratory).

Control stands for cells without any compound, incubated in 0.1 mM CaCl₂ and 0.1 M KCl at 25°C. Control had an average proton efflux rate (n mol/mg yeast cells/min) of 5.6, 5.1, 6.1, 6.3, and 5.9 respectively for C. albicans, C. kruesi, C. guillermondii, C. tropicalis, and C. glabrata, respectively. Results are records of 3–4 independent sets of experiments. Fluconazole was the positive control.

The data (mean ± SD) represent three sets of experiments.