Synthesis strategies for optimizing sizes of PLGA nanoparticles containing recombinant Chenopodium album (rChe a 3) allergen

ABSTRACT

The size of antigen delivery systems impact on the immune induction. The purpose of this study was to obtain poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) containing rChe a 3 allergen in different sizes (about 200, 400, and 800 nm). The optimal conditions were obtained by adjusting different parameters comprising molecular weight/concentration of polyvinyl alcohol, solvent type (dichloromethane or dichloromethane/acetone), sonication time, and addition of cryoprotectant (sucrose). The optimized formulations were found to be stable for 60 days at 4°C. In conclusion, the preparation conditions need to be adjusted to obtain allergen-PLGA NPs with specific aforementioned sizes.

GRAPHICAL ABSTRACT

KEYWORDS:

• Allergen
• encapsulation efficiency
• PLGA
• preparation conditions
• size