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ABSTRACT
A poly(hemin-co-C16-co-TEGDA) monolith was prepared using hemin and 1-hexadecene (C16) as monomers; triethylene glycol dimethacrylate (TEGDA) as crosslinking agent; N,N-dimethylformamide and isopropyl alcohol as porogens; and dibenzoyl peroxide and N,N-dimethyl aniline as initiators in 50 × 4.6 mm i.d. stainless steel column. The morphology and structure of the monolith were characterized by scanning electron microscopy and nitrogen adsorption/desorption isotherm measurement, respectively. Compared with poly(C16-co-TEGDA) monolith, poly(hemin-co-C16-co-TEGDA) monolith had better mechanical properties, higher permeability, and better separation ability. The prepared monolith was used to separate not only proteins but also small molecules.
GRAPHICAL ABSTRACT
Additional information
Funding
The study was funded by National Natural Science Foundation of China (No. 21175031, 21575033, 21505030), the Natural Science Foundation of Hebei Province (No. B2015201024, B2015201085), and the Natural Science Foundation of Hebei University (No. 2014-05).