Biogenic PHA nanoparticle synthesis and characterization from Bacillus subtilis NCDC0671 using orange peel medium

ABSTRACT

The present research work was initiated to synthesize biogenic polyhydroxyalkanoate (PHA) nanoparticles from Bacillus subtilis NCDC0671 using orange peel medium. Sequential and systematic optimization of bioprocess variables was performed using “one-factor-at-a-time” approach for maximizing PHA yield. Maximum PHA yield was achieved under optimized conditions of temperature (37°C), pH (7), carbon source (sucrose), and nitrogen source (beef extract) on 72 h of fermentation using B. subtilis NCDC0671. PHA nanoparticles were synthesized using triple emulsion method and conjugated with drug levofloxacin. Dialysis method was used to evaluate the in vitro drug releasing efficacy of PHA–levofloxacin nanoparticles.

GRAPHICAL ABSTRACT

KEYWORDS:

• Bacillus subtilis
• drug delivery system
• levofloxacin
• orange peel medium
• polyhydroxyalkanoates

Acknowledgments

The authors thank Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu for providing the necessary facilities for the successful execution of this research work. The authors thank STIC, Cochin University of Science and Technology, Kochi, Kerala for NMR and TGA facilities. The authors also acknowledge Department of Nanoscience and Technology, Bharathiar University, Coimbatore, Tamil Nadu for providing FESEM and XRD facilities.

Additional information

Funding

The authors declare that this research work has not received any specific grants from funding agencies in the public, private or commercial sector.