Immunomodulatory properties of D-sorbitol/D-mannitol incorporated linear step-growth Co-polymers

Abstract

A range of D-sorbitol/D-mannitol incorporated co-polymers were synthesized via solvent free enzyme catalyzed polycondensation with adipic acid and polyethylene glycol. In-vitro cytotoxic studies on breast cancer and fibroblast cell lines showed that the co-polymers are biocompatible up to 500 and 250 µg/mL, respectively. However, THP-1 and RAW 264.7 cell lines showed a dose dependent cytotoxicity. Furthermore, these co-polymers did not produce excessive cytokines (TNFα/IL-6) and chemokines (CXCL-5/CXCL-10) when interacting with THP-1 cells, thereby, not causing any inflammation. Whilst, the expression of IL-1b, CXCL-1 and IFNβ were upregulated. The results suggest that these co-polymers could be promising vaccine adjuvants.

Graphical Abstract

Keywords:

• D-mannitol/D-sorbitol based co-polymers
• toxicity studies
• immunomodulatory effects
• vaccine adjuvant)

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was funded by the Science and Engineering Research Board (SERB), Department of Science and Technology, Govt. of India under the Fast Track Scheme for young scientists [No: SB/FT/CS-129/2012]. The authors are thankful to HLL Lifecare Limited, Trivandrum, Kerala, India for providing necessary facilities and support. KBH is supported by RGCB-laboratory research fund.