Characterization of isoniazid incorporation into chitosan-poly(aspartic acid) nanoparticles

Abstract

The incorporation of the drug Isoniazid (an anti-tuberculosis drug) into nanoparticles of polyelectrolyte chitosan-poly(aspartic acid) complexes was carried out by the ionotropic gelation technique, adding a sodium polyaspartate solution containing the drug to a chitosan solution in different proportions in a mass ratio (mg/mg) Isoniazid/Chitosan. This was confirmed by MALDI-TOF/MS, ESI/MS, FTIR, and UV-Vis. The lowest average particle diameter (PD), estimated by DSL, was 148.5 ± 1 nm corresponding to an Isoniazid/chitosan ratio (mg/mg) of 0.5:1. FE-SEM showed nanoparticles in the order of 100–300 nm; whereas AFM showed semi-spherical particle morphology.

Graphical Abstract

Keywords:

• Incorporation
• ionotropic gelation
• isoniazid
• nanoparticles
• polyelectrolyte complex

Acknowledgments

The authors wish to thank Iván Olortiga, Aurica P. Chiriac, Kevin Tucker, Franco Basile, Rong Zhou, Hugo Alarcón, Alcides López, Silvia Suarez, and Dolores Rivera for their helpful contribution to different aspects of this research.

Author contributions

Carmen L. Zegarra-Urquia: investigation, methodology, conceptualization, formal analysis, and writing—original draft preparation. Julio Santiago: supervision, resources, and writing—original draft preparation. Joel Bumgardner, Alexander Goroncy, and José Vega-Baudrit: formal analysis and writing—original draft preparation. Claudia A. Hernández-Escobar: validation. E. Armando Zaragoza-Contreras: supervision, writing, review, and editing.

Disclosure statement

No potential conflict of interest was reported by the author(s).