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Abstract
Two new Ru(II) complexes, [Ru(dmp)2(dadppz)](ClO4)2 (1) and [Ru(dip)2(dadppz)](ClO4)2 (2), were synthesized and characterized by elemental analysis, ES-MS, and 1H NMR. DNA-binding behaviors were investigated by absorption titration and luminescence spectra. The DNA-binding constants were 3.1 (± 0.2) × 104 and 3.0 (± 0.2) × 105 M−1 for 1 and 2. The antioxidant activity of these complexes against hydroxyl radical () was explored. The cytotoxicities in vitro towards A549, BEL-7402, MG-63, and SKBR-3 cells were studied by 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Complex 1 shows high cytotoxicity, but 2 is not cytotoxic towards the selected cell lines. Apoptosis and cell cycle distribution were studied by flow cytometry. The cellular uptake showed that complexes can enter into the cytoplasm. JC-1 was used as a fluorescence probe in detecting the mitochondrial membrane potential and results indicate that the mitochondrial membrane potential decreases.
Acknowledgments
This work was financially supported by the National Nature Science Foundation of China (No. 31070858) and Guangdong Pharmaceutical University.
