Immobilization of glucose oxidase based on the sodium alginate-modified products of a functionalized metal organic framework and the application for one-pot analysis of glucose

Abstract

A self-assembled functionalized metal-organic framework, Zn₄O(NH₂BDC)₃ microcrystalline (IRMOF3) could be prepared by washing complex with DMF and CH₂Cl₂. Via modification with sodium alginate (AA), IRMOF3-AA samples could be obtained. IR and TEM were used for structural confirmation of IRMOF3-AA samples. PEG-GOD could be obtained via PEGylation of glucose oxidase (GOD), which could be confirmed by X-ray photoelectron spectroscopy (XPS) and used for further application. IRMOF3-AA@PEG-GOD samples could be obtained by the immobilization of PEG-GOD to IRMOF3-AA, and the best immobilization condition was achieved by adjusting the amount of GOD. From the view point of enzyme mimics, combined with peroxidase-like properties of the IRMOFS-AA samples, the determination of glucose was demonstrated with a linear range from 5 × 10⁻⁵ mol/L to 1.6 × 10⁻³ mol L⁻¹ using tetramethylbenzidine (TMB) as chromogenic substrate. The prepared IRMOF3-AA@PEG-GOD samples exhibited dual functional enzyme-like (peroxidase and GOD-like) activities, with simple experimental steps.

Keywords:

• IRMOF3
• PEG-GOD
• Modification
• Mimicking bi-enzyme system

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

This work was supported by the Research Project of Tianjin Education Commission (grant no. 2017KJ190), Outstanding Research Achievement Award Project of Tianjin Agricultural University, the Open Topic of Guangxi Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (CMEMR2016-B12), the National Natural Science Foundation of China (Nos. 21875117 and 31572492), the National-level Innovative and Entrepreneurial Training Plan for College Students (201710061035), the Veterinary Biotechnology Scientific Research Innovation Team of Tianjin, China (grant no. TD12-5019), the Veterinary “Innovative Talent People of Young and Middle Age Key Members Project” of Tianjin, the Natural Science Foundation of Tianjin (grant no. 18JCYBJC30100).