Three Cd(II) coordination polymers induce gastric cancer cell apoptosis by promoting intracellular ROS accumulation and apoptotic genes expression

Abstract

In this study, three new Cd(II) coordination polymers, [Cd(TPT)(NO₃)₂(MeOH)](CH₂Cl₂) (1), [Cd₃(TPT)₂(NO₃)₆(MeOH)₄](MeOH)₂ (2) and [Cd(TPT)(NO₃)₂] (3), with structural diversity [from one-dimensional (1D) chain to 3D framework structures] have been prepared by using 2,4,6-tris-(pyridyl)-1,3,5-triazine (TPT) as ligand. The CCK-8 assay was used to explore the anti-proliferation effect of 1–3 on MKN45 gastric cancer cells, Annexin V-FITC/PI assay was performed to confirm the apoptosis and cell cycle arrest of MKN45 in a flow cytometer; the intracellular ROS level was also determined in this study. mRNA expressions of p53, nf-κb, and tnf-α in MKN45 cancer cells after treatment with 1–3 was measured by quantitative reverse transcription polymerase chain reaction. The biological results showed that 1 exerted anticancer activity through inducing apoptosis by upregulating the apoptotic genes and intracellular ROS level.

Keywords:

• Coordination polymer
• structural diversity
• proliferation
• gastric cancer
• apoptosis
• ROS

Disclosure statement

No potential conflict of interest was reported by the authors.