SEPARATION AND PREPARATION OF VALIDAMYCIN A AND VALIDAMYCIN B USING ANION-EXCHANGE RESIN

Abstract

A method for the separation and preparation of validamycin A and validamycin B by anion-exchange resin is described. The two compounds, validamycin A and validamycin B, could be separated effectively in the 10 × 400 mm column with anion-exchange resin A600 under the optimized conditions: elution flow rate of 0.25 mL/min, added quantity of 0.7 g, bed height of 330 mm, and deionized water as eluent. The yields of validamycin A and validamycin B were 58.9% and 89.5%, respectively.

Keywords:

• Anion-exchange resin
• Separation
• Validamycin A
• Validamycin B
• Validamycins

Acknowledgments

This work was supported by Zhejiang Provincial Natural Science Foundation of China (ZB0106), National Natural Science Foundation of China (20176055), and National 973 Project of China (2003CB114402).