OPTIMIZATION OF LIPASE-CATALYZED SYNTHESIS OF N-trans-FERULOYLTYRAMINE USING RESPONSE SURFACE METHODOLOGY (RSM)

Abstract

Enzymatic synthesis of N-trans-feruloyltyramine amide was optimized by response surface methodology (RSM) using 4-hydroxy-3-methoxycinnamic acid and tyramine hydrochloride in a one-step lipase catalyzed reaction using Lipozyme TL IM. Response surface methodology (RSM) based on five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interaction of synthesis, reaction time (24–96 h), temperature (30°–50°C), amount of enzyme (50–500 mg, 12.5–125.0 IUN), and substrate molar ratio (cinnamic acid:tyramine HCl) 1:1–8:1 mmol on the percentage yield of N-trans-feruloyltyramine amide. The optimum conditions derived via RSM were: reaction time 52 h, temperature 43°C, amount of enzyme 260 mg (65.0 IUN), and substrate molar ratio (cinnamic acid:tyramine HCl) 6.2:1. The actual experimental yield was 96.3% under optimum conditions, which compared well to the maximum predicted value of 97.2%.

Keywords:

• Cinnamic acid
• Enzymatic synthesis
• Lipozyme
• N-trans-feruloyltyramine
• RSM
• Tyramine HCL

Notes

^a Significant at Prob > F less than 0.05 and insignificant at Prob > F more than 0.05.

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