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Abstract
The scarlet (Solanum aethiopicum) and gboma (S. macrocarpon) eggplants are two African crops with potential for vegetable crop diversification in subtropical climates. Here we carried out the in vitro regeneration in two accessions of each species. In a comparison of five media, we found that those containing the cytokinin thidiazuron (TDZ) induced the greatest regeneration response from both cotyledonary and true leaf explants. After organogenesis induction, explants were transferred to Murashige & Skoog (MS) basal medium without growth regulators for 20 days. Isolated shoots rooted after culture on MS medium for 4–6 days. Optimisation of TDZ concentration was studied in a range of concentrations from 0.05 to 0.8 μM. In general, increases in the concentration of TDZ in the medium resulted in a higher density of buds, but they had a slower development. Although differences in regeneration ability were observed among varieties, TDZ concentrations of 0.1 or 0.2 μM gave the best results, with 70–100% explants with shoots and a mean of 2–7 shoots per explant. The efficient regeneration obtained for these species allows the propagation of important genotypes and their improvement through in vitro techniques.
