![Sample our Environment & Agriculture journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5934/TOC-Subject-Sample-2021-Environment-Agriculture.jpg"})
ABSTRACT
A highly efficient in vitro micropropagation protocol, using newly emerged sprouts coming from greenhouse-grown plants, was developed to produce Actinidia melanandra Franch. and Actinidia rubricaulis Dunn. This is the first report of in vitro culture of A. rubricaulis Dunn. Axenic culture was possible using one- or two-node explants or terminal buds, surface-sterilised with commercial bleach tablets. Nearly 90% success rate in establishment was observed. The best axillary shoot proliferation and maximum adventitious shoots elongation were achieved on Quoirin-Lepoivre medium supplemented with 1.5 mg L−1 6-benzyladenine and 30 g L−1 sucrose. Although used as an inert support, agar brand has a significant effect. Kobe agar at 8 g L−1 gave the best response. 100% of in vitro rooting was observed. Roots length and their quality were highly improved by the use of vermiculite in transfer medium. Over 99% of rooted plants survived after acclimatisation.
Acknowledgements
The author sincerely thanks Sylvie Parkinson for the revision of the manuscript as well as INRA Grande Ferrade (Bordeaux, France) for providing plants from which cuttings were realised in order to produce the starting material for the in vitro establishment. Sylvain Teissonnière is particularly thanked for his picture on acclimatised plants. This research was supported by funds from the CASDAR 2012–2015, France.
Disclosure statement
No potential conflict of interest was reported by the author.