The improved antiviral activities of amino-modified chitosan derivatives on Newcastle virus

Abstract

Chitosan is widely used as a medical material because of its excellent biological activities. However, the low solubility of natural chitosan limited its medicinal activity to some extent. The solubility can be improved by introducing more active groups and lowering molecular weight. Therefore, 6-amine chitosan derivatives were synthesized in this paper since more active groups were introduced to increase the medicinal activity. Those derivatives were characterized by elemental analysis, HPLC, and FT-IR and the antiviral activity was tested by hemagglutination tests. Finally, 6-amine chitosan derivatives improved the antiviral activity, especially after the introduction of bromine ion. When 6-deoxy-6-bromo-N-phthaloyl chitosan was 1 g/L, they reduced the hemagglutination titer of virus to zero. The RT-PCR result showed that the expression level of TNF-α and IFN-β increased significantly, which indicated that the antiviral activity of amino-modified chitosan worked through the stimulation of immune response.

Keywords:

• 6-Deoxy-6-bromo-N-phthaloyl chitosan
• antiviral activity
• Newcastle virus
• mechanism assumption

Acknowledgements

The authors gratefully thank Dr. Yanbo Yin and their research group in Qingdao Oland Better Bioengineering Co., Ltd. for providing instrumentation and technical assistance.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by National Key R&D Program of China [2018YFC0311305]; Special projects of Foshan science and technology innovation team [2017IT100054].