Protective effects of diarylpropionitrile against hydrogen peroxide-induced damage in human neuroblastoma SH-SY5Y cells

Abstract

Oxidative stress is implicated in pathogenesis of neurodegenerative disorders, such as Alzheimer’s and Parkinson’s diseases. The study demonstrates diarylpropionitrile (DPN), an antioxidant selective agonist of estrogen receptor β, protected human neuroblastoma SH-SY5Y cells against H₂O₂-induced toxicity by attenuating production of reactive oxygen species, apoptosis, autophagy, NF-κB activation, MAPK p38, JNK and ERK 1/2 signaling pathways, and β-site amyloid precursor protein cleaving enzyme level, but, interestingly, stimulating Akt pathway. These findings indicate the important potential of DPN to ameliorate oxidative stress-associated damage in neurodegenerative disorders.

Keywords:

• Alzheimer’s disease
• diarylpropionitrile
• hydrogen peroxide
• neuroblastoma SH-SY5Y cells
• oxidative stress

Acknowledgements

The authors thank Professor Prapon Wilairat for critical reading of the manuscript, which was proof-read by Mahidol University Graduate School manuscript editing service

Conflicts of interest

The authors declare no conflicts of interest.

Additional information

Funding

The research was partially supported by a joint Mahidol University and The Thailand Research Fund (TRF) grant (IRG5780009) and the 60^th Year Supreme Reign of His Majesty King Bhumibol Adulyadej Scholarship of the Faculty of Graduate Studies, Mahidol University.