Mechanistic study of copper oxide, zinc oxide, cadmium oxide, and silver nanoparticles-mediated toxicity on the probiotic Lactobacillus reuteri

Abstract

The use of metal/metal oxide nanoparticles (NPs) in consumer products has increased dramatically. Accordingly, human exposure to these NPs has increased. Lactobacillus reuteri, a member of the beneficial gut microbiota, is essential for human health. In the present study, the toxic effect of three metal oxides (CuO, ZnO, and CdO) and one metal (Ag) NPs on L. reuteri were investigated in vitro. L. reuteri was susceptible to all the prepared NPs in a dose-dependent manner, visualized as an increase in the zones of inhibition and a significant reduction in the maximum specific growth rates (µ_max). The minimal inhibitory concentrations were 5.8, 26, 560, and 560 µg/mL for CdO-, Ag-, ZnO-, and CuO-NPs, respectively, and the respective minimal bactericidal concentrations were 60, 70, 1500, and 1500 µg/mL. Electron microscopic examinations revealed the adsorption of the prepared NPs on L. reuteri cell surface, causing cell wall disruption and morphological changes. These changes were accompanied by significant leakage of cellular protein content by 214%, 191%, 112%, and 101% versus the untreated control when L. reuteri was treated with CdO-, Ag-, CuO-, and ZnO-NPs, respectively. NPs also induced oxidative damage, where the malondialdehyde level was significantly increased, and glutathione content was significantly decreased. Quantifying the DNA damage using comet assay showed that CuONPs had the maximum DNA tail length (8.2 px vs. 2.1 px for the control). While CdONPs showed the maximum percentage of DNA in tail (15.5% vs. 3.1%). This study provides a mechanistic evaluation of the NPs-mediated toxicity to a beneficial microorganism.

Keywords:

• Cadmium oxide nanoparticles
• copper oxide nanoparticles
• zinc oxide nanoparticles
• silver nanoparticles
• Lactobacillus reuteri
• nanotoxicity

Author contributions

The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. AE: Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, and Writing original draft. OMS: Data curation, Methodology, Supervision, Writing - reviewing and editing. WH: Supervision, and Writing - reviewing and editing. TD: Conceptualization, Data curation, Methodology, Project administration, Supervision, Writing original draft, and reviewing and editing

Disclosure statement

No potential conflict of interest was reported by the authors.