Isolation of Gram Quantities of Configurational Isomers of Cyclic Nitrosamines by Preparative Liquid Chromatography

Abstract

Substituted cyclic nitrosamines, e.g., 2,6-dimethyl-N-nitrosomorpholine and 3,5-dimethyl-N-nitrosopiperidine are separable into their component configurational isomers by preparative hplc. Gram quantities of the individual isomers sufficient for animal testing were obtained. The identification of contaminants in commercial 2,6-dimethylmorpholine of purported 99% is discussed.