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Abstract
Chemically bonded phases have been prepared using thermally treated silicagels as basic material. Four bonding reactions with trimethylchlorosilane (TMCS) were successively performed on each silicagel in order to obtain the maximum coverage.
The effect of the chemical modification on the surface structure of packings and on their retention behaviour in reversed-phase chromatography was investigated. The maximum coverage with trimethylsiloxy groups was observed with silicagels previously treated at 300–400°C while the minimum concentration of hydroxyl groups was calculated for silicagels previously treated at 600°C.
Significant differences in retention were observed for these phases in reversed-phase chromatography. Silicagels with maximum coverage were found to be the most convenient for the analysis of apolar solutes while silicagels with minimum hydroxyl group concentration were the most convenient for the analysis of polar solutes.