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Abstract
The present paper describes a sensitive and precise method for the determination of methimazole in plasma of patients with hyperthyroidism under carbimazole therapy.
The method uses the 2–6 dichloroquinone chloroimide which forms a complex with methimazole sulfur group. After selective chloroform extraction of the complex, high performance liquid chromatography (HPLC) on a μNH2 column was performed in the reconcentrated extract. The drug was detected with a spectrophotometer at a wavelength of 405 nm. The sensitivity of the method allowed the detection of a concentration as low as 5 ng ml−1 of plasma. No interference with other biological products was detected.