Silver Ion as Modifier for High Performance Liquid Chromatography of Menaquinones

Abstract

The chromatography of the homologous series of menaquinones with 0 to 10 isoprenoid units on reverse phase high performance liquid chromatography is investigated with octyldecylsilica and C-8 silica supports. Addition of chloroform decreases retention (k¹) of all menaquinones and vitamin K₁ linearly with respect to log k¹. The addition of silver ion decreases k¹ of menaquinones with 3 or more isoprenoid units with a non-linear relationship to log k¹. There is a linear relationship at a given silver ion concentration between log k¹ and the number of isoprenoid units. There is no effect of silver ion on vitamin K₁, although there is one double bond in the side chain.