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Abstract
High pressure liquid chromatography (HPLC) methods were developed for separation and plasma analysis of ten xanthine derivates. Separation was evaluated on silica column and on three different reverse phase columns, with optimum conditions obtained on C6 spherisorb column using isocratic elution with phosphate buffer 10−2 M, pH 2.7 - acetonitrile mixture (80/20 V/V). Determination of these xanthine derivates in plasma for therapeutic control was studied.