High-Speed Gel Permeation Chromatography of Human Thyroglobulin and Sheep Liver Aldehyde Dehydrogenase

Abstract

This paper reports the application of high speed gel permeation techniques for the fractionation of human thyroglobulin preparations on chemically bonded, polar phase microparticulate silicas. Both resolution and recovery were found to be dependent on the ionic strength and pH of the eluent. At high salt concentrations significant hydrophobic interactions may occur between the support matrix and the protein solutes. The sheep liver enzyme, aldehyde dehydrogenase, was found to exhibit similar salt-and pH-dependent effects under these chromatographic conditions.