Reverse Phase Ion Pair High Performance Liquid Chromatography of Viral Tryptic Glycopeptides

M. C. Kemp Departments of Microbiology and Medicine, University of Alabama Medical Center, Birmingham, Alabama, 35294, U.S.A.

W. L. Hollaway Departments of Microbiology and Medicine, University of Alabama Medical Center, Birmingham, Alabama, 35294, U.S.A.

R. L. Prestidge Departments of Microbiology and Medicine, University of Alabama Medical Center, Birmingham, Alabama, 35294, U.S.A.

J. C. Bennett Departments of Microbiology and Medicine, University of Alabama Medical Center, Birmingham, Alabama, 35294, U.S.A.

R. W. Compans Departments of Microbiology and Medicine, University of Alabama Medical Center, Birmingham, Alabama, 35294, U.S.A.

Abstract

Tryptic glycopeptides from influenza A/WSN (H₀N₁) and mink cell focus (MCF)-inducing (MCF-247) murine leukemía virus were subjected to high performance liquid chromatography (hplc) for mapping purposes. Hydrophilic ion-pairing was accomplished using 0.1% phosphoric acid on C₁₈ μBondapack and LiChrosorb RP-18 ODS commercially available columns. The samples were eluted from the column with acetonitrile with sample recovery in the range of 70 to 80%. The advantages of hplc over the conventional techniques previously used are detailed.

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Reverse Phase Ion Pair High Performance Liquid Chromatography of Viral Tryptic Glycopeptides

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Reverse Phase Ion Pair High Performance Liquid Chromatography of Viral Tryptic Glycopeptides

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