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Abstract
A specific method for the quantitation of digoxin in serum has been developed and applied to determination of drug concentrations in serum from digitalized patients. One ml of the supernatant from acetonitrile denatured serum, subsequently diluted with water, was chromatographed on a reversed phase HPLC column. The eluent corresponding to the retention time of digoxin was collected and analysed using a commercial radioimmunoassay kit. The recovery at 3ng/ml was 96.1 ± 3.0%. Endogenous substances, drugs and metabolites of digoxin do not interfere with the method. The mean value of the samples estimated by the present method was 84% of those determined by direct R.I.A.