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Original Articles

Assay of Brain Tocopherols Using High Performance Liquid Chromatography

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Pages 109-121 | Published online: 05 Dec 2006
 

Abstract

The four natural tocopherols were separated using a μ-Bondapak-NH2 column. For the analysis of brain tocopherols 5,7-dimethyltocol was used as an internal standard. α-Tocopherylquinone and other tocopherols than α-tocopherol were not detected. Rat cerebral cortex and cerebellum contained 19.3 μmol/g and 11.2 μmol/g of α-tocopherol, respectively. A chromatographic system with a reversed-phase column proved less suitable.

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