High-Resolution Anion-Exchange Chromatography of Ultraviolet-Absorbing Constituents of Human Erythrocytes

Abstract

The analysis of nucleic acid components of human erythrocytes was achieved by high-resolution anion exchange chromatography using a column packed with a macroreticular anion-exchange resin and linear gradient elution with ammonium acetate solution, pH 4.5. On the chromatogram of trichloroacetic acid extracts from human erythrocytes, at least 50 components were detected as ultraviolet-absorbing constituents. On assignment of the chromatographic peaks they were found to be nucleic acid components. The analysis was achieved in 120 min and the elution time of guanosine 5′-triphosphate was 64.3 min.