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Abstract
An experimental study of the gel permeation chromatography of a series of poly (sodium styrene sulfonates) in aqueous media has been undertaken. The objective of this study was to develop a set of operating conditions that would maximize peak separation and minimize peak broadening. Using CPG-10 porous glass packing, it has been found optimal to work at pH near 6 and ionic strength near 0.05. Under those conditions, ion exclusion is eliminated and the optimum balance between resolution and reproducibility is obtained. Addition of a few ppm neutral surfactant to the mobile phase prevents adsorption to the packing surface, and improves resolution.