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Abstract
Fatty alcohols were obtained from the saponified chloro-form-methanol extract of Mycobacterium tuberculosis H37Ra by extraction with diethyl ether and partially purified by silicic acid column chromatography. The long-chain fatty alcohols (C49-C58) were separated from the shorter-chain alcohols by Sephadex LH-20 column chromatography and further fractionated into saturated and unsaturated alcohols by argentation thin-layer chromatography. These two fractions were analyzed by proton nuclear magnetic resonance spectro-scopy, derivatized to the 3,5-dinitrobenzoyl esters, and fractionated on a C18-bonded silica column by high performance liquid chromatography (HPLC). Complete separation of esters differing by two carbon units and partial separation