High Performance (Pressure) Liquid Chromatography Separation and Quantification of Picomole Amounts of Prostaglandins Utilizing a Novel Triethylamine Formate Buffer

D. M. Desiderio Department of Neurology, University of Tennessee Center for the Health Sciences, 800 Madison Avenue, Memphis, Tennessee, 38163; Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Center for the Health Sciences, 800 Madison Avenue, Memphis, Tennessee, 38163

M. D. Cunningham Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Center for the Health Sciences, 800 Madison Avenue, Memphis, Tennessee, 38163; Department of Toxicology, VA Hospital, Memphis, Tennessee, 38104

J. A. Trimble Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Center for the Health Sciences, 800 Madison Avenue, Memphis, Tennessee, 38163

Abstract

A novel, volatile buffer system utilizing dilute triethylamine-formic acid is useful for high resolution HPLC separation and quantification of nanogram amounts of prostaglandins. Lyophilizability of the buffer facilitates subsequent mass spectral, bioassay or radioimmunoassay analyses.

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High Performance (Pressure) Liquid Chromatography Separation and Quantification of Picomole Amounts of Prostaglandins Utilizing a Novel Triethylamine Formate Buffer

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High Performance (Pressure) Liquid Chromatography Separation and Quantification of Picomole Amounts of Prostaglandins Utilizing a Novel Triethylamine Formate Buffer

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