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Abstract
An enzymatic detection of urinary steroid-17β-glucuronides is described. The principle of the method is as follows; after gel filtration with Sephadex G-25 β-glucuronidase is added to each effluent fraction and incubated for 20 h at 37 $C. After hydrolysis, 3β, 17β-hydroxysteroid dehydrogenase is added and incubated for 20 min at 37 $C. An absorbance at 500 nm is read aginst sample of first fraction effluent.