Iodination of Peptide Hormones and Purification of Iodinated Peptides by HPLC

Abstract

Hexagastrin, Arg⁸-vasopressin, oxytocin, Tyr¹-somatostatin, and ACTH 1-39 were iodinated in order to yield precursors for tritium labelling or radioiodinated tracers for radioimmunoassay, respectively. The heterogeneous mixture of iodination products was purified via reversed-phase high-performance liquid chromatography. Iodination of peptides resulted in a marked increase in retention time on the reversed-phase adsorbent. A simple and quick method was applied for purification of radioiodinated peptides on a Sep-pak® C-18 cartridge for rapid sample preparation.