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Abstract
Two-dimensional/2D/ chromatography was first described by Kirchner in 1951. A sample applied to one corner of a 20 × 20cm plate approximately 1.5cm from each edge is developed in a mobile phase that resolves the polar constituents. It is then dried, rotated 90 degrees to the first direction and placed in a second mobile phase designed to resolve the more non-polar components. In this manner, a multitude of diverse compounds can be resolved.