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Abstract
Herein is reported an experimental investigation of the molecular weight characterization of dextrans by aqueous SEC with a DRI/LALLSP detector system. Methodology for the determination of the molecular weight calibration curve and peak broadening parameter [sgrave]2 (variance of a Gaussian instrumental spreading function) across the chromatogram have been developed. A blend of dextran standards permits, with one injection, measurement of the molecular weight calibration curve over a wide range of retention volumes. Measurements with salt-free water as mobile phase have confirmed that some dextran chains may have a negative charge.