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Abstract
Two types of high performance aqueous size exclusion columns have recently been developed, one a rigid spherical silica-based packing containing a new hydrophilic bonded phase (MicroPak TSK Gel Type SW) and the other an organic-based, semi-rigid gel (MicroPak TSK Gel Type PW). Characteristics of MicroPak TSK SW and PW columns were compared to other commercially available aqueous SEC columns packed with similar supports. Chromatographic performance of prepacked columns containing microparticulate support materials were compared for exclusion separations of water-soluble organic polymers, biopolymers, and small water-soluble oligomers. Amino acid probes were used to investigate non-exclusion effects of MicroPak TSK SW and PW columns.