Rapid Separation of Testosterone and its Microsomal Metabolites by Reverse-Phase High Performance Liquid Chromatography

J. F. Newton Department of Pharmacology and Toxicology Center for Environmental, Toxicology Michigan State University East Lansing, Michigan, 48824

W. E. Braselton Department of Pharmacology and Toxicology Center for Environmental, Toxicology Michigan State University East Lansing, Michigan, 48824

L. F. Lepper Jr. Department of Pharmacology and Toxicology Center for Environmental, Toxicology Michigan State University East Lansing, Michigan, 48824

K. M. Mccormack Department of Pharmacology and Toxicology Center for Environmental, Toxicology Michigan State University East Lansing, Michigan, 48824

J. B. Hook Department of Pharmacology and Toxicology Center for Environmental, Toxicology Michigan State University East Lansing, Michigan, 48824

Abstract

High performance liquid chromatography has been used to separate testosterone from its oxidative metabolites (7α-, 16α -and 6 β-hydroxytestosterone and androstenedione) following in vitro incubation with rat liver microsomes. The separation was accomplished in less than 18 minutes on a radially compressed C₁₈ reverse-phase column using isocratic elution with tetra-hydrofuran: water (24/76, v/v).

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Rapid Separation of Testosterone and its Microsomal Metabolites by Reverse-Phase High Performance Liquid Chromatography

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Rapid Separation of Testosterone and its Microsomal Metabolites by Reverse-Phase High Performance Liquid Chromatography

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