Chromatographic Separation on Silica of Polar Aromatic Compounds II) Research of the Best Chromatographic System by Different Preloading in Thin Layer for a Transposition on Column

Abstract

A high performance thin layer chromatographic method for aromatic compounds separation with absorbant deactivation has been given in the precedent work. Our aim was to transpose the results obtain from HPTLC to HPLC. The two types of results were then related by a transposition coefficient, K_tR which interprets the geometrical variations of the two chromatographic methods. The values obtained for K_tR represent fairly well the linear relation between the retentions in thin layer chromatography and those in column chromatography. We have thus desmontrated the possibility of modifying adsorbent activity in the same manner in thin layer as in column chromatography by the fixation of chemical compounds, thus allowing the separation of relatively polar compounds.