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Abstract
This communication reports on our recent application of size-exclusion high performance liquid chromatography for the rapid analysis of insulin and monitoring the progress of its enzymatic hydrolysis. Insulin, a protein hormone, exhibits a complex association behavior in solution. Consequently, analysis of insulin and its derivatives by conventional methods is difficult. We are able to analyze insulin and some of its derivatives, and also tc study the trypsin-proteolysis of insulin by using size-exclusion high performance liquid chromatography under that denaturing condition of 7 M urea. This analytical method is rapid, reproducible, selective and simple.