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Abstract
A method for the simultaneous measurement of acetaminophen and salicylate by reverse phase HPLC using N,N-Diethyl-m-toluamide as internal standard is described. The method, which utilizes a one step acetonitrile precipitation of serum, can be applied to as little as 25ul. of sample. Serum is Vortex-mixed with acetonitrile containing the internal standard and following centrifugation an aliquot of the supernatant is analyzed on a reverse-phase column. The eluting solvent consists of methanol/5% acetic acid (60/40, V/V) and the effluent is monitored at 248 nm.