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Abstract
TLC is a potentially powerful technique for the separation of surfactants. Reversed phase thin layer chromatography (RPTLC) can be used to separate entire classes of surfactants (i.e., anionics from nonionics from cationics). Conversely, silica gel can be used to separate individual anionic or cationic surfactants from other similarly charged surfactants. RPTLC can also be used to separate individual nonionic surfactants. Using two dimensional TLC (with a special silica gel plate containing a 2.5 cm strip of reversed phase material along one edge) a complex mixture of surfactants was first fractionated into classes and then (using the second dimension) into individual components. Standard scanning densitometry was used for quantitation.