Chromatography of Amino Acids on Reversed Phase Thin Layer Plates

Abstract

The separation of 19 amino acids was studied on reversed phase thin layers, including C₁₈ chemically bonded silica gel, impregnated silica gel, and acetylated cellulose. Normal aqueous-organic solvents, aqueous micellar solutions, and reversed micellar solutions were tested as mobile phases. The only practical system that provided a reversal in migration sequence compared to silica gel and cellulose included a C₁₈ layer impregnated with HDBS, and this reversal was apparently due to an ion exchange mechanism.