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Abstract
A simple radioisotopic method is described for the determination of glutamic acid decarboxylase in biological materials. It is based on the direct determination of γ-amino-butyric acid produced by the incubation of glutamic acid decarboxylase with the radiolabelled substrate. Separation is achieved by thin-layer chromatography on Dowex 1-acetate coated plastic strips. The assay is linear for GABA production with both time and enzyme concentration. The method was also used to determine the presence of glutamic acid decarboxylase in bovine subretinal intercellular fluid and retina.