Exclusive Use of High Pressure Liquid Chromatography for the Determination of the Complete Amino Acid Sequence of the 12K Fragment of Avian Sarcoma Virus Structural Protein p27

Ajit S. Bhown Department of Medicine, Division of Clinical Immunology and Rheumatology Birmingham, Department of Microbiology University of Alabama in Birmingham, Alabama, 35294

J. Claude Bennett Department of Medicine, Division of Clinical Immunology and Rheumatology Birmingham, Department of Microbiology University of Alabama in Birmingham, Alabama, 35294

Thomas W. Cornelius Department of Medicine, Division of Clinical Immunology and Rheumatology Birmingham, Department of Microbiology University of Alabama in Birmingham, Alabama, 35294

Eric Hunter Department of Medicine, Division of Clinical Immunology and Rheumatology Birmingham, Department of Microbiology University of Alabama in Birmingham, Alabama, 35294

Abstract

Using exclusively high pressure liquid chromatography for the protein and peptide separation complete primary structure of the 12,000 molecular weight (12K) amino terminal (1–87 residues) fragment obtained by mild acid hydrolysis of p27 (Avian Sarcoma Virus structural protein) has been determined. The sequence was established by direct degradation of the native molecule and its (12K) peptides isolated by molecular exclusion and reverse phase HPLC.

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Exclusive Use of High Pressure Liquid Chromatography for the Determination of the Complete Amino Acid Sequence of the 12K Fragment of Avian Sarcoma Virus Structural Protein p27

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Exclusive Use of High Pressure Liquid Chromatography for the Determination of the Complete Amino Acid Sequence of the 12K Fragment of Avian Sarcoma Virus Structural Protein p27

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