A Simple and Rapid Method for the Determination of 2-Acetylaminofluorene in Laboratory Diets

E. G. Perkins Department of Food Science, University of Illinois, 1208, West Pennsylvania Urbana, Illinois, 61801

D. J. Hendren Department of Food Science, University of Illinois, 1208, West Pennsylvania Urbana, Illinois, 61801

C. R. Truex Clinical Nutrition and Metabolism, University of Illinois College of Medicine Urbana, Illinois, 61801

W. J. Visek Clinical Nutrition and Metabolism, University of Illinois College of Medicine Urbana, Illinois, 61801; Department of Food Science

Abstract

A simple and rapid method for the determination of 2-AAF in animal feeds was developed using high performance liquid chromatography. The column employed was an octadecyl bonded silica support with 80% methanol as mobile phase. Fluorene was added as an internal standard. The method is applicable for concentrations of 250–1200 μg AAF/kg diet.

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A Simple and Rapid Method for the Determination of 2-Acetylaminofluorene in Laboratory Diets

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A Simple and Rapid Method for the Determination of 2-Acetylaminofluorene in Laboratory Diets

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