Dispersion-Compensated Calibration for Gel Permeation Chromatography: Theory and Utilization for Direct and Universal Calibration

Abstract

This paper describes the error involved in the utilization of nonmonodisperse polymer standards for the calibration of GPC columns. It also demonstrates that the computer technique developed by McCrackin (1) produces a calibration equation from poly-disperse standards that avoids this error. This equation, referred to as dispersion-compensated, can successfully be incorporated into the theory of universal calibration to remove the necessity of assuming infinite instrumental resolution. Furthermore, it is shown that only the calibration equation compensated for number-average molecular weights is a true universal form.