Concentration Dependence of Elution Volumes in Size Exclusion Chromatography of Polymer Molecules. 1. Effect of Viscosity and of Coil Contraction in Good Solvent

Abstract

The contribution from viscosity phenomena and coil size contraction to the shift of polymer elution volumes with increasing concentration has been evaluated in size exclusion chromatography through a practical experimental procedure. It is shown that the viscosity effect is operative to different extents, depending on the different column systems. For most of the investigated polymer samples, however, macromolecular coil contraction seems to be the main contributing effect to the total concentration dependence of polymer elution volumes.