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Abstract
An ion pair reversed-phase HPLC method was developed for the determination of flourescent DNS-AA and by-products DNS-OH, DNS-NH2, and DNS-N(CH3)2 which form during the dansylation reaction. The method permits the separation of the four fluorescent compounds and ultraviolet absorbing DNS-C1 in 25 min. Dansylation of amino acids was carried out under different reaction conditions and evaluated with regard to major products formed and not only the DNS-AA. Optimum reaction conditions can readily be obtained for the dansylation of amino acids using this method.