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Abstract
Separation of catechol estrogen monoglucuronides and mono-sulfates by high-performance liquid chromatography with electrochemical detection on a reversed-phase column has been carried out. The effects of composition and pH of mobile phases on the capacity factor were investigated with a TSKgel ODS-120T column. Each group of isomeric monoglucuronides and monosulfates of 2- and 4-hydroxyestrogens was efficiently resolved on this column when the 0.5% ammonium dihydrogen phosphate-tetrahydrofuran-acetonitrile system was used as a mobile phase.