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Abstract
Identification and quantitative determination of phomenone in ethyl acetate extracts from Tomato plants infected by Phoma destructiva Plowr., was carried out by high-performance liquid chromatography with UV detection, and ethanol-water (30/70, v/v) as mobile-phase on Perkin-Elmer RP-18/10 stainless column, at 20°C and 0.9 ml min−1 flow rate. Detection limit was 5.5 ng, with standard deviation of ± 3%, and retention time of 6.22 min. Analysis of extracts from spiked tomato fruits shows the same parameters. The method appears to be adequate for detection and quantitation of phomenone in contaminated tomato leaves and fruits using a pre-column packed with Lichroprep RP-18/25–40 μm.